解剖学报

›› 2010, Vol. 41 ›› Issue (1): 104-108.doi: 10.3969/j.issn.0529-1356.2010.01.020

• 论著 • 上一篇    下一篇

内皮素-1在烫伤大鼠脑血管损伤中的表达

林雪群1* ;戴育成2;祝高春1;万丽丹1   

  1. 1. 南昌大学医学院脑血管研究所，解剖学教研室，南昌 330006;2.南昌大学第二附属医院分子医学重点实验室，南昌 330006
  • 收稿日期:2008-05-12 修回日期:2008-08-28 出版日期:2010-02-06
  • 通讯作者: 林雪群

Expression of endothelin-1 in cerebral vessels of scalded rats

  1. 1.Institute of Cerebral Vessels Research, Department of Anatomy, Medical College of Nanchang University, Nanchang330006, China;2.Molecular Medical Center of the Second Affiliated Hospital, Nanchang University，Nanchang330006,China
  • Received:2008-05-12 Revised:2008-08-28 Online:2010-02-06
  • Contact: LIN Xue-qun

关键词: 内皮素-1, 烫伤, 脑动脉, 免疫印迹法, 反转录-聚合酶链反应, 放射免疫分析, 大鼠

Abstract: Objective To investigate the expression and effects of endothelin-1 (ET-1) in cerebral vessels of scalded rats. Method Histological method was used to observe the alteration of histological structure of basilar artery in 8 scalded rats. Reverse transcription polymerase chain reaction(RT-PCR) was performed to examine expression of ET-1 mRNA; Western blotting to analyse expression of endothelin-1; radioimmunoassay to measure ET-1 levels, respectively, of cerebral basilar artery in 48 scalded rats. Result Vascular pathohistological changes were observed in cerebral basilar artery after rats were scalded; ET-1, ET-1 mRNA expression and ET-1 levels of basilar artery in scald 6 hours, 12 hours and 24 hours group were obviously higher than that of control group. Conclusion Scald may cause an increases of ET-1 expression in basilar artery, and elevated ET-1 expression may relate to the pathogenesis of cerebral vascular injury in scalded rats.

Key words: Endothelin-1, Scald, Cerebral artery, Western blotting, RT-PCR, Radioimmunoassay, Rat

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